Data/hora: 01/06/2024 00:19:05

Biblioteca(s): | Biblioteca Rui Tendinha.
Data corrente: | 05/07/2020
Data da última atualização: | 05/07/2020
Autoria: | MAURASTONI, M.; SÁ-ANTUNES, T. F.; OLIVEIRA, S.; SANTOS, A. M. C.; VENTURA, J. A.; FERNANDES, P. M. B.
Afiliação: | Marlonni Maurastoni, UFES; Tathiana F. Sá?Antunes, UFES; Scarlett A. Oliveira, UFES; Alexandre M. C. Santos, UFES; Jose Aires Ventura, Incaper; Patricia M. B. Fernandes, UFES.
Título: | A multiplex RT-PCR method to detect papaya meleira virus complex in adult pre-flowering plants.
Ano de publicação: | 2020
Fonte/Imprenta: | Archives of Virology, v. 165, p. 1211-1214, july 2020.
Idioma: | Inglês
Conteúdo: | Papaya sticky disease (PSD), which can destroy orchards, was first attributed to papaya meleira virus (PMeV). However, the discovery of papaya meleira virus 2 (PMeV2) associated with PSD plants impose the need to detect this viral complex. We developed a multiplex RT-PCR (mPCR) technique capable of detecting two viruses in a single assay from pre-flowering plant samples, which is a useful tool for early diagnosis of PSD. We also determined the limit of detection (LOD) using asymmetric plasmid dilutions of both PMeV and PMeV2, which revealed that a higher titer of one virus prevents detection of the other. Thus, this technique is an alternative method for detecting PMeV and PMeV2 in a single reaction.
Palavras-Chave: | Doença das Planta; Multiplex; Papaya meleira virus (PMeV).
Thesagro: | Carica papaya; Doença de planta.
Categoria do assunto: | H Saúde e Patologia
Registro original: | Biblioteca Rui Tendinha (BRT)
Exemplares: | BRT - (23438-1) - UMT - AP - DD -  -  -  - DIS